In this lab you will compare three different methods to dispense liquids, a burette, a pipette and a micropette (also called a pipetteman). We will use the average value of 10 measurements to obtain the statistics appropriate to determination of the mean and standard error. For the micropipette you will determine a calibration line. This is a appropriate since a micropipette is adjustable and it is typically used to dispense a range of different volumes.

Laboratory protocol

The goal of this initial lab is partly to test your skill in pipetting and weighing accurately, but more importantly to demonstrate the use of linear regression to create a calibration line. We will apply this to the calibration of a micropipette. This type of pipette is widely used in laboratories and these instruments lose their calibration rather quickly. They depend very sensitively on the suction used to draw up a defined volume of liquid.

Laboratory instructions in a PDF document (from manual)